Table 2. The p53RE-related sequence that is predicted to have the greatest function in the promoter regions (±5 kb from transcription start site) of human TLR genes.
| Gene | Total number p53RE related sequences * | Predicted most functional p53 RE | Chromosome coordinates** | Conserved in rodents | Tested in reporter assay |
| TLR1 | 11 (2) | RE#4 AGGCATGagC-tAcCATGCCC | 4: 38807969-38807950 | NO | YES |
| TLR2 | 10 (3) | RE#4 AAACAAGaTCtacccctatatGGGCATGTCC | 4: 154621975-154622005 | NO | YES |
| TLR3 | 13 (2) | RE#4 AGGCATGCaC-cAACATGCCC | 4: 186988380-186988399 | NO | YES |
| TLR4 | 15 (4) | RE#8 AGGCATGCTCcaGAGCAAaTCT | 9: 120464256-120464277 | NO | YES |
| TLR5 | 11 (3) | RE#4 GGGCATGgTg-GYACATGCCT∧ | 1: 223311442-223311461 | NO | YES |
| TLR6 | 11 (4) | RE#6 RAGCAAGTCCactGGCAAaTCTggGGGCATGTga∧ | 4: 38832459-38832492 | NO | YES |
| TLR7 | 5 (2) | RE#2 GGGCATGTCacaatttcagattaatcaAcGCTTGCTC | X: 12885856-12885892 | NO | NO |
| TLR8 | 10 (7) | RE#6 AGGCAAGaTg-AAACATRTCa∧ | X: 12923665-12923684 | NO | YES |
| TRL9 | 17 (3) | RE#14 AGGCATGgTgGtGCATGCCT | 3: 52273914-52273933 | NO | YES |
| TLR10 | 6 (1) | RE#4 AGACATGTTT-GtAtATGTTT | 4: 38786796-38786815 | NO | YES |
In lower case characters are the mismatches relative to the consensus p53 response element (RE).
The bold characters are half-site p53REs without mismatches relative to the consensus p53 RE.
The italic lower case characters correspond to the spacer sequence between p53 decamers. A lack of spacer between decamer sequences is denoted by “-”.
∧ Indicates that the predicted RE contains a single nucleotide polymorphism (SNP). SNPs are indicated in bold and underline characters.
TLR5 SNP rs2192617 C/T
TLR6 SNP rs167251 G/A
TLR8 SNP rs3761624 A/G
*Number of noncanonical p53 REs is indicated inside the parenthesis.
**Chromosome coordinates based on USC Genome Browser Human Feb. 2009 (GRCh37/hg19) http://genome.ucsc.edu/.