Skip to main content
. 2011 Mar 31;7(3):e1002039. doi: 10.1371/journal.pgen.1002039

Figure 4. Alteration of V. cholerae N16961 motility and biofilm formation upon expression of wild-type or mutated putative DGCs and PDEAs from C. difficile 630.

Figure 4

(A) Relative motility upon expression of wild-type or mutated CD0757 or CD1420. (B) Relative biofilm expression upon expression of wild-type or mutated CD0757 or CD1420. (C) Motility of the strain expressing the wild-type (WT) and mutated proteins on LB soft agar plates at 30°C. The picture shown is representative of four independent experiments. (D) Schematic representation of CD0522 protein fragments expressed. The amino acids in the signature motifs of the GGDEF or EAL domains are indicated above each domain. Positions of the mutations in the GGDEF or EAL domains are indicated by asterisks. (E) Relative motility upon expression of full-length, mutated or truncated CD0522. (F) Relative biofilm formation upon expression of full-length, mutated or truncated CD0522. The means and standard deviations obtained from four independent assays are shown. One-way ANOVA with a Dunnett multiple comparison post-test was used to compare the cells over-expressing putative c-di-GMP signalling proteins and the control strain (LacZ) (*, P<0.05; **, P<0.01; ***, P<0.001).