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. 2011 Mar 31;7(3):e1001358. doi: 10.1371/journal.pgen.1001358

Figure 3. Expression patterns of AGO10, AG, and WUS in floral meristems.

Figure 3

(A, B) Immunolocalization of AGO10 protein using anti-GFP antibodies in zll-1 pZLL::YFP-ZLL flowers. The grey signals represent AGO10. Before floral stage 6, AGO10 was predominantly localized at the floral meristem and in provascular tissue (A); AGO10 was adaxialized in carpels beyond stage 6 (B). (C, D) The ago10-13 mutation did not alter the spatial patterns of AG expression in developing flowers. AG mRNA was similarly present in the inner two whorls of an ag-10 (C) and an ag-10 ago10-13 (D) flower at stages 6–7. (E–H) ago10 mutations led to prolonged WUS expression. In hua1 hua2, the latest stage when WUS mRNA could be detected was stage 6 (E). WUS expression persisted to stages 8-9 in hua1 hua2 ago10-12 flowers (arrow in F). WUS expression was diminished by stage 7 in ag-10 (G). In ag-10 ago10-13, WUS expression could be observed in stages 8–9 flowers (arrow in H). Scale bars, 50 µm. ca, carpel; se, sepal; st, stamen.