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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Anal Chem. 2011 Mar 8;83(7):2598–2604. doi: 10.1021/ac102929y

Figure 3. Thrombin-stimulated serotonin release.

Figure 3

(A) Washed platelets release more serotonin at a faster rate when stimulated with increasing concentrations of thrombin (symbol ▼). (B) Release trace analysis includes the determination of [5-HT]max, slope (initial rate normalized to the saturating [5-HT]max ), Tdelay, and T50%. (C-E) Quantitative analysis of stimulation and secretion coupling. Measurements were performed at 37 ± 1 °C in platelet suspensions of 2.0 × 108 platelets/mL. Data are represented as mean ± SD, N = 3 for all measurements.