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. Author manuscript; available in PMC: 2011 Sep 30.
Published in final edited form as: Oncogene. 2010 Dec 6;30(13):1586–1596. doi: 10.1038/onc.2010.540

Figure 7. UVB induced apoptosis in primary mouse keratinocytes.

Figure 7

Near-confluent primary mouse keratinocytes were irradiated with different doses of UVB, and control cells were sham-irradiated. The cells were lysed 24 hrs post-UVB exposure and processed for the caspase-3 activity assay as described in Methods. Values are expressed relative to the control and represent the means ± SEM of 3 independent experiments performed in triplicate; **p<0.05 versus the control by ANOVA followed by a Dunnett’s post-hoc test. (B) Samples were processed for western blotting employing an antibody recognizing PARP to monitor its cleavage (a marker of apoptosis and caspase-3 activation). Actin served as the loading control. Illustrated is a blot representative of 3 separate experiments.