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Colorimetric determination of EpCAM expression using EpCAM antibody–Protein G-PNC conjugate. TMB was used as the substrate. (a) At pH 7.0, absence of substrate oxidation was observed, as TMB is a poor substrate at this pH.25 (b) Expression of EpCAM was determined, due to the oxidation of TMB by nanoceria at pH 4.0, with a detection limit of 3600 cells.
