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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Anal Chem. 2011 Mar 3;83(7):2547–2553. doi: 10.1021/ac102826k

Scheme 1.

Scheme 1

Schematic showing the HRP/H2O2 and nanoceria mediated oxidation of ampliflu. In the pH range 4–7, HRP/H2O2 oxidizes Ampliflu to a non-fluorescent final product (Resazurin). (a) In contrast, nanoceria oxidizes Ampliflu to the intermediate oxidation fluorescent product (Resorufin) at pH 7 (b), while at or below pH 5.0, nanoceria yields the terminal oxidized non-fluorescent product resazurin. (c). The ability of nanoceria to oxidize ampliflu to a stable fluorescent product in the pH range 6–8, will facilitate its use in ELISA. (d, e) without the use of H2O2.