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Phospholipids inhibit solute permeation through fatty-acid-based membranes. (A) Permeability of C10 membranes (4∶1∶1 DA∶DOH∶GMD) to ribose as a function of DDPA content as measured by a stopped-flow relaxation assay. (B) Leakage of encapsulated ImpdA from C10 vesicles as measured by scintillation counting of dialysis buffer aliquots. Membrane compositions: □, 4∶1∶1 DA∶DOH∶GMD; ▴, 4∶1∶1 DA∶DOH∶GMD with 25 mol % DDPA; and *, DDPA.
