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. 2011 Mar 8;108(13):5449–5454. doi: 10.1073/pnas.1019007108

Fig. 3.

Fig. 3.

Early diet- and aging-associated effects on transcriptional activity and epigenetic regulation at the Hnf4a locus in rat islets. (A) mRNA analysis by qRT-PCR in 3M and 15M C and LP samples (3M, n = 10 per group; 15M, n = 8 per group). Data were normalized against Ppia and are shown relative to 3M C, arbitrarily set to 1. (B) MassArray analysis of DNA methylation at the P2 promoter in 3M and 15M C and LP samples (n = 6 per group). Native ChIP analysis of histone marks at the enhancer (C) and P2 promoter (D) in 3M and 15M C and LP samples (3M, n = 7 per group; 15M, n = 6 per group). Note that the levels of H3K4me3 were not compared between C and LP islets at the enhancer because of its very low levels at this region (Fig. 1D). H3K4me1 was not compared at the P2 promoter because it is a mark for active enhancers (23). Data are shown relative to the 3M C samples, arbitrarily normalized to 1. Error bars indicate SEM. *P < 0.05; **P < 0.01; ***P < 0.001.