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. 2011 Mar 8;108(13):5449–5454. doi: 10.1073/pnas.1019007108

Fig. 4.

Fig. 4.

Long-range interactions at the rat Hnf4a locus. (A) Schematic representation of the 300-kb region on rat chromosome 3 containing the Hnf4a locus, analyzed by q3C. Green arrows indicate the transcriptional orientation of the genes within this region. Blue vertical bars indicate CTCF/cohesin-binding sites validated by ChIP in rat liver. (B) Comparative P2-enhancer association frequency in adult liver (n = 4), INS-1 cells (n = 5), and adult islets (n = 3). (C) Proposed looping models that summarize the long-range interactions in islets and liver. Red circles indicate CTCF/cohesin-binding sites, and blue circles indicate sites not bound by CTCF/cohesin. (D) P2-enhancer association frequency in 3M C (n = 4) and LP samples (n = 5). Data are shown relative to the 3M C samples, arbitrarily normalized to 1. 3M C products after 33 PCR cycles are shown. Error bars represent SEM. *P < 0.05; **P < 0.01.