Fig. 4.

Reduced Mps1 levels result in decreased in vivo growth of breast cancer xenografts. (A–C) Growth of mouse xenografts tumors of Hs578T cells with doxycycline-inducible Mps1 shRNA compared with xenografts with cells from parental (C) and vector (no dox) (A and C) controls. In this experiment, doxycylcine treatment began 24 h after inoculation of tumor cells. Error bars indicate SEM. (D) Weights of tumors explanted from animal killed 20 d after inoculation. Error bars indicate SEM, and P values were calculated by using Student's t test. (E) Western blot analysis of Mps1 protein and BubR1 protein using lysates from tumors of control (parental), vector, and Mps1 shRNA xenografts, compared with MCF10A cells as a frame of reference. (F) Quantitation of Mps1 and BubR1 proteins from tumors normalized to controls, with P values calculated by using Student's t test.