WT KcsA, but not E71A, becomes nonconductive in the absence of K+ and the presence of Na+. (A) Time courses of WT fluxes at pH 7, showing K+-driven uptake (black), K+-driven uptake (blue), and Na+-driven uptake (orange) (n = 3, mean ± SEM). (B) Same as A except for E71A (n = 3, mean ± SEM).