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Relocalization to the TGN restores copper transport activity of ATP7B-Arg875. (A) Colocalization with TGN38 (red) staining confirms efficient targeting of Flag-ATP7B-Arg875 (green) to the TGN in the YSTT cells following treatment with 5 μM copper. (Scale bar: 14 μm.) (B) In the tyrosinase assay, pigment formation is observed in cells transfected with ATP7B-Arg875 and treated with 5 μM copper; control cells treated with 5 μM copper show no color.
