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. Author manuscript; available in PMC: 2011 Sep 30.
Published in final edited form as: Oncogene. 2010 Nov 29;30(13):1566–1576. doi: 10.1038/onc.2010.532

Figure 3. Inhibiting α5β1-integrin, silencing c-Met or both blocks invasion which can be overcome by activation of c-Met by HGF.

Figure 3

(a) HeyA8 cells were transfected with the indicated siRNAs and seeded on matrigel coated Boyden chambers, treated with human α5Ab and allowed to invade for 24 h. Silencing was confirmed by immunoblotting. (b) HeyA8 cells or HeyA8 cells transfected with c-Met siRNA were placed in matrigel coated Boyden chambers, treated with human α5Ab with or without HGF/SF or untreated control as indicated and allowed to invade for 24h. For (a) and (b) the average number of invaded cells/field was plotted against the treatment given. Results are from three independent experiments.