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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: Dev Biol. 2011 Jan 27;352(2):393–404. doi: 10.1016/j.ydbio.2011.01.023

Figure 3.

Figure 3

Characterization of Tg(otpb.A:egfp)zc48; confocal images of whole mount embryos double-labeled for GFP and for TH immunohistochemistry, ventral views, anterior to the top. Scale bar is 50 μm. (A) Schematic diagram of TH-positive cell groups in the zebrafish brain at 72hpf, based on Rink and Wullimann, 2002. (B) Confocal z-stack projection of TH immunohistochemistry at 72hpf in the zebrafish brain. (C) Confocal z-stack projection of GFP immunohistochemistry in Tg(otpb.A:egfp)zc48. (D-D″) Confocal z-stack projections at different dorsal-ventral levels in the brain of Tg(otpb.A:egfp)zc48 at 72hpf, showing co-expression of TH and GFP in diDA neuron groups 4 and 6, but not groups 1 and 2 (arrow); arrowhead points to the NPO neurons. (E-E″) Magnified views of the region boxed in B″, showing extensive overlap of GFP-positive neurons in the diencephalon with TH expression. (F-F″) Expression at 24hpf (G-G″) Expression at 48hpf.