Fig. 3A–C.

The FACS plots show no detectable antibody production in PVG rats transplanted with either frozen bone from PVG (syngeneic) or PVG.1U (allogeneic MHC mismatched) rats. The final serum dilution was 1/8. Propidium iodide was used for gating on live cells. The plots show (A) FITC labeled secondary Ab alone (gray filled), mAb OX6 against MHC-II (thin line) and a standard antibody against MHC-I of the RT1u haplotype mAb NR5/10 (thick line), (B) counts for three pretransplanted rats, and (C) from two representative rats of each group (syngeneic [gray filled and stapled line] and allogeneic [thin and thick lines]).