Figure 2.

Quantitative analysis of the decellularization of DM1 utilizing (a) calcein uptake (n = 3) and (b) DNA quantification (n = 6). Fluorescent microscopy images of calcein uptake pre- (c) and post-decellularization (f). Bright field images (100× magnification) of cell layers pre- (d) and post-decellularization (g). SEM images of cell layers pre- (e) and post-decellularization (h) at 8172× magnification. Scale bars represent 250 μm (c, d, f, g) and 2 μm (e, h). *p < 0.0001 vs. pre-decell layers (a, b)