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hMSCs cultured on DM1 exhibited increased calcium deposition compared to cells on DM2 or control substrates. (a) Calcium deposited from hMSCs cultured on each substrate for 1, 3, and 5 weeks. Alizarin red staining of fixed hMSC layers cultured for 3 weeks on (b) DM1, (c) DM2, (d) FN, and (e) TCP. # p < 0.05 vs. all groups, † p < 0.05 vs. FN and TCP (n = 3–4)
