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Impact of pIV upon ArcAB activity. The impact of pIV upon ArcAB activity was assessed using a ϕpfl–lacZ reporter fusion to the ArcA-P dependent pflB promoter (pGJ46). pIV has a modest effect on pflB promoter activity. Wild-type: MG1655; ΔpspF: MG1655ΔpspF; ΔarcB: MG1655ΔarcB (MVA59). pIV was under leaky expression on pGJ4 (no addition of IPTG). Cells were grown microaerobically as described in “Material and Methods”
