Table 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristics | Reference |
|---|---|---|
| Strain | ||
| BW25113 | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), &lambda −, rph-1, Δ(rhaD-rhaB)568, hsdR514 | CGSC# 7636 |
| MC1061 | F-, Δ(araA-leu)7697, [araD139] B/r, Δ(codB-lacI)3, galK16, galE15(GalS), &lambda −, e14-, mcrA0, relA1, rpsL150(strR), spoT1, mcrB1, hsdR2 | CGSC# 6649 |
| MG1655 | F-, lambda −, rph-1 | CGSC# 7740 |
| RP437 | F-, thr-1, araC14, leuB6(Am), fhuA31, lacY1, tsx-78, lambda −, eda-50, hisG4(Oc), rfbC1?, rpsL136(strR), xylA5, mtl-1, metF159(Am), thi-1 | CGSC# 12122 |
| MG1655ΔpspF | F-, lambda −, rph-1, ΔpspF | [30] |
| MVA4 | MC1061ϕpspA-lacZ (apr) | [16] |
| MVA44 | MG1655ϕpspA-lacZ (apr) | [25] |
| MVA59 | MG1655ΔarcB::kan (kanr) | [25] |
| BT3415 | RP437Δtsr-7021 nuoF::miniTn10cm (cmr) | [14] |
| BT3390 | RP437Δtsr-7021 ndh:erm (ermr) | [14] |
| BT3402 | BT3415Δ(cyoABCDE)456::kan (kanr) | [14] |
| BT3406 | BT3390Δ(cyoABCDE)456::kan (kanr) | [14] |
| BT3408 | BT3390 Zbg-2200::kanΔ(cydAB′)455 (kanr) | [14] |
| EC10 | RP437 ϕpspA-lacZ (apr) [RP437×MVA4] | This study |
| EC11 | RP437Δtsr-7021 nuoF::miniTn10cm (cmr) Δ(cyoABCDE)456::kan (kanr) ϕpspA-lacZ (apr) [BT3402×MVA4] | This study |
| EC12 | RP437Δtsr-7021 ndh:erm (ermr) Δ(cyoABCDE)456::kan (kanr) ϕpspA-lacZ (apr) [BT3406×MVA4] | This study |
| EC13 | RP437Δtsr-7021 ndh:erm (ermr) Zbg-2200::kanΔ(cydAB′)455 (kanr) ϕpspA-lacZ (apr) [BT3408×MVA4] | This study |
| JW3901 | BW25113ΔmenA::kan (kanr) | [28] |
| EC14 | MC1061ϕ pspA-lacZ ΔmenA::kan (kanr) [MVA4×JW3901] | This study |
| Plasmids | ||
| pGZ119EH | IPTG-inducible tac promoter expression vector (cmr) ColD origin of replication | A gift from M. Russel |
| pPMR129 | pGZ119EH harbouring gIV (pIV); ColD origin of replication; (cmr) | A gift from M. Russel |
| pGJ4 | IPTG-inducible Ptac promoter expression vector encoding gIV (pIV); ColD origin of replication; (tetr) | [25] |
| pGJ46 | 490 bp of pflB promoter region amplified by PCR from the chromosome of MG1655 and cloned into pRS415A via EcoRI–BamHI; p15A origin of replication; (apr) | [26] |
| pGJ66 | 193 bp of yfiD promoter region amplified by PCR from the chromosome of MG1655 and cloned into pRS415A via EcoRI–BamHI; p15A origin of replication; (apr) | This study |
Listed are the strains and plasmids used in this study