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Reversible interaction of SUMO1 and Ubc9 in living cells. (a) FRET assays of stable HEK293 cell line containing CyPet–SUMO1 and YPet–Ubc9 after different treatments with or without protein interaction disrupting reagents, such as Triton-100 or Urea. (b) Plot of FRET ratio (Em530/Em475) of cells treated with or without disrupting buffers as in (a). (c) Positive hit selection of seven hundred screened compounds
