Figure 1.
Quantification of surface adsorption of fibronectin (FN) at different densities. (a) Representative images of glass coverslips coated with FN at theoretical densities of 0.1, 1.0, and 10.0 μg/cm2, respectively, and probed with FN antibody (scale bar = 20 μm). (b) Intensity analysis of surface-adsorbed FN at different theoretical densities. Box–whisker plots of median intensities (10 random positions per condition) at different theoretical densities. (c) Quantification of surface-adsorbed FN by Western blot. FN adsorbed to glass coverslips at different densities was extracted and run on a blot to determine the amount of attached FN. Quantification of the band intensities by densitometry revealed a saturating trend with a fivefold increase in surface-adsorbed FN between 0.1 and 1.0 μg/cm2