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Effect of LPA on cortical stiffness of U373 MG cells cultured on different density FN substrates under no-serum conditions. Box–whisker plots of single cell stiffness of U373 MG glioma cells cultured on different density FN substrates with or without LPA treatment. Cell stiffness remained unchanged across all densities and upon LPA treatment on 0.1 and 1.0 μg/cm2 substrates. However, on 10.0 μg/cm2 substrates, LPA treatment led to a significant (red bar) increase in cell stiffness
