Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jan 31;10(4):M110.002642. doi: 10.1074/mcp.M110.002642

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Fig. 5. — CBX proteins do not interact with each other and do not colocalize. A, GST-pull down analysis of interactions between CBX4 and CBX family proteins indicates that CBX4 oligomerizes but does not interact directly with the other CBX proteins in vitro. GST-tagged CBX4 and GFP control proteins were expressed in E. coli, bound to glutathione-Sepharose, and incubated with in vitro expressed TAP-tagged CBX proteins as indicated. Bound material was washed, proteins were separated by SDS-PAGE and Western blots probed with a peroxidase anti-peroxidase (αPAP) antibody to reveal TAP-tagged proteins. B, CBX proteins show different nuclear localization patterns. U2-OS cell lines stably expressing GFP-tagged CBX proteins were fixed with paraformaldehyde and localization of fusion proteins visualized by fluorescence microscopy (right panels). Hoechst staining labels nuclei (left panels).