Fig. 2.

Evaluation of assay performance for 4 × 24-plex homogenous PLA. Exemplified by data for the detection of interleukin 6(IL-6) where the recombinant antigens are present at concentrations ranging from 2–200 pm in buffer. To illustrate specificity, assay evaluation was also performed in smaller antigen subset mixes selected from all antigens in a panel for which IL-6 was only present in mix #3. Assay specificity was also assessed in 100% chicken plasma. Linearity of the assay was further tested by performing two fivefold dilutions in PBS with 0.1% BSA from 100% to 4% plasma and recovery experiments calculated by spiking 20 pm pure antigen in plasma, data normalized against GFP.