Table II. Configuration of the four panels used for multiplex PLA biomarker screening.
Panel | 1 | 2 | 3 | 4 |
---|---|---|---|---|
Dilution | 1:1 | 1:1 | 1:10 | 1:100 |
Sequence | ||||
1 | Carbonic Anhydrase IX | Survivin | Spondin-2/Midin | Clusterin |
2 | MCP-1 (CCL2) | Desmin | EPCAM | No assay |
3 | Mesothelin | HGF R/cMET | NSE (enolase 2) | sP-selectin |
4 | IL-6 | IL-17 | Transaldolase 1 | MMP-2 |
5 | No assay | IL-23 | No assay | MMP-9 |
6 | oit1/FAM3D | FoxP3 | Galectin-3 | Tenascin-C |
7 | CEACAM 5 pAb | CEACAM 5 pAb | TIMP1 | TIMP1 |
8 | VEGF | CEACAM1 | EGFR | CRP |
9 | No assay | TGFb1 | TFF3 | PAI-1/Serpin |
10 | No assay | TGFb3 | u-PA | sVCAM/CD106 |
11 | CEA mAb | KLK3 (PSA) | CEA mAb | IGF-I |
12 | CA125 | Cystatin SN | Transferrin Rec. (CD71) | Cathepsin B |
13 | Her4 | Prolactin | CA 15.3 | Angiogenin |
14 | HGF/SF | DcR3 | CA19–9 | IGF2 |
15 | FABP2 | IL8 | Tropomyosin (CH1) | Tetranectin |
16 | Oligo PCRa | Oligo PCRa | Oligo PCRa | Oligo PCRa |
17 | APCa | APCa | APCa | APCa |
32 | GFPa | GFPa | GFPa | GFPa |
35 | IL8 | CTGF | OPN | Cystatin C |
38 | PEa | PEa | PEa | PEa |
45 | Her2 | GDNF | MIF | IGFBP-1 |
51 | Her3 | Fractalkine | No assay | GRP78/HSPA5/BiP |
53 | TNFa | MIP-1 | SLPI | Kallikrein 11 |
57 | IL-1a | CA 242 | S100A8 | YKL-40 |
a Internal control used for normalization and reaction quality control.