Table III. PLA performance.
| Panel | 1 | 2 | 3 | 4 | Total |
|---|---|---|---|---|---|
| Assay detects protein in healthy plasma (total) | 14 (17) | 14 (20) | 16 (18) | 16 (19) | 60 (74) |
| Number of assays with available antigen that lie within estimated sensitivity range belowa | |||||
| 10 fM - 100 fM | 1 | 2 | 1 | 1 | 5 |
| 100 fM - 1 pm | 10 | 6 | 2 | 5 | 23 |
| 1 pm - 10 pm | 3 | 4 | 2 | 6 | 15 |
| 10 pm - 100 pm | 1 | 1 | 3 | 2 | 7 |
| CV inter-sample variation of PE (n = 148 samples) | |||||
| Raw | 52% | 67% | 44% | 54% | |
| Normalized (GFP) | 18% | 18% | 10% | 11% | |
| Recovery of spiked-in PE detection (median) | |||||
| Raw | 20% | 24% | 70% | 114% | |
| Normalized (GFP) | 149% | 170% | 84% | 119% | |
a See Fig. 3 for details.