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Percent inhibition of T3-induced TRE-luciferase activity in HEK293 cells. A, T3-mediated transcription is reduced by MSNB analogs. HEK293 cells were transfected with CMV-TRβ- and DR4-TRE-driven luciferase expression vectors. The data were normalized to Renilla luciferase activity. B, cell viability based on Renilla luciferase activity. Error bars represent S.D. of two independent experiments performed in triplicate. **, p < 0.01; ***, p < 0.005.
