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Time course of covalent acylation of PFK-1 with [14C]palmitoyl-CoA. Purified rabbit muscle PFK-1 (1 μm) was incubated with 10 μm [1-14C]palmitoyl-CoA at 35 °C in 25 mm Tris-HCl, pH 7.5, containing 50 mm KCl and 1 mm DTT for the indicated times. Samples were resolved by SDS-PAGE and visualized by autoradiography as described under “Experimental Procedures.” Results presented are representative of at least three separate experiments.
