FIGURE 1.

Schematic of E2F1 constructs. A schematic of the various E2F1 fusion protein constructs is shown. All constructs contain the influenza HA tag and the estrogen receptor ligand binding domain (ER) immediately preceding the N terminus (indicated as -N) of the E2F1 coding sequence. The amino acid positions of the different domains are indicated above each construct. These domains include the cyclin A binding domain (CycA), the DBD, the heterodimerization domain (Dimer), the marked box domain (MB), and the C-terminal (indicated as -C) transactivation domain (TAD) with the pocket protein binding domain (pRB) embedded within it. The E2F1ΔMB is unique in that it has the SV40 large T antigen nuclear localization domain (NLS) introduced immediately upstream of the E2F1 coding sequence. For the E2F1 DBD mutant, the two vertical bars within the DBD domain represent a two-point amino acid substitution.