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. 2011 Feb 12;286(14):12024–12032. doi: 10.1074/jbc.M110.168625

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Twist and Snail1 cooperate in Zeb1 expression. 5 μg of TWIST1 hshRNA1 or Non-Target Control Vectors were transfected to RWP-1 Snail1 (panel A), SW-620 or MiaPaca-2 cells (panel B). Transfected cell populations were selected with puromycin (2.5 μg/ml), and analyzed by quantitative (A) or semi-quantitative RT-PCR (B). Similar results were obtained with another TWIST1 shRNA (hshRNA2) in RWP-1 Snail1 cells that inhibited ZEB1 expression by 55%. Panel C, RWP-1 cells were transfected with expression plasmids containing Twist or Snail1 cDNAs; cell populations were selected with G418 and analyzed by RT-PCR for the expression of the indicated genes. Panel D, RWP-1 cells were transiently transfected with the indicated cDNAs and pGL3-ZEB1 promoter. Luciferase activity was determined after 48 h. The figure shows the average ± S.D. of the results (D) or a representative experiment of three performed (A, B, and C).