FIGURE 5.

p38 MAPK activation in IL-32α-induced Fas and ULBP2 expression. IL-32α-overexpressing K562 cells were incubated with 40 μm SB203580 (SB) for 48 h. After incubation time, the cells were stained with PE-conjugated anti-human Fas (CD95) Ab and PE-conjugated anti human ULBP2 Ab. A, Fas and ULBP2 expression by histogram analysis. B, K562 cells were transiently transfected with vector (pcDNA3.1⁺) or IL-32α cDNA (IL-32α/pcDNA3.1⁺). Αfter 24 h, K562 vector transfectants and IL-32α transfectants were lysed with cell lysis buffer as described under “Experimental Procedures.” Whole protein lysates were assessed with KU32–52 (mouse anti-human IL-32), rabbit anti-phosphor-p38 MAPK, and rabbit anti-total p38 MAPK. Data presented are representative of three independent data sets for each condition.