TABLE 1.

Parameters related to elongation and breakage rates determined by fitting of solution cross-seeding experiments

The values shown were derived from the global fit (see “Experimental Procedures”) of the data of cross-seeding experiments (as displayed in Fig. 5) and the errors shown are the standard error of the global fit of each experiment. ξ₁ = k₊/L₀ and ξ₂ = k₊ k₋, where k₊ and k₋ are the rate constants for elongation and breakage, respectively, and L₀ is the average number of molecular units in a seed fibril, which will be constant for a given batch of seed.

Seed	ScUre2p
Soluble protein	ScUre2p		SpUre2p
Parameter	ξ1	ξ2	ξ1	ξ2
	s−1·m−1	10−6·s−2·m−2	s−1·m−1	10−6·s−2·m−2
Batch
I	3.4 ± 0.2	2.4 ± 0.3	8.0 ± 0.5	1.2 ± 0.3
II	6.7 ± 0.4	2.7 ± 0.2	14.6 ± 0.6	2.5 ± 0.2
III	5.7 ± 0.2	2.1 ± 0.2	10.3 ± 0.5	1.1 ± 0.2

Seed	SpUre2p
Soluble protein	ScUre2p		SpUre2p
Parameter	ξ1	ξ2	ξ1	ξ2
	s−1·m−1	10−6·s−2·m−2	s−1·m−1	10−6·s−2·m−2
Batch
I	2.4 ± 0.1	1.7 ± 0.1	6.3 ± 0.3	2.2 ± 0.4
II	11.7 ± 0.5	3.9 ± 0.6	27.3 ± 1.3	7.9 ± 2.9
III	4.9 ± 0.2	5.4 ± 0.3	11.2 ± 0.5	6.5 ± 0.7