FIGURE 3.

Epo increased TJ protein ZO-1 expression in human fetal immature IEC. A and C, H4 immature IEC were grown on tissue culture plates and treated with 0, 0.1, 0.5, and 1 unit/ml recombinant human Epo for 24 h. Total cell lysates were collected and subjected to immunoblotting for ZO-1 and β-actin in A and ZO-2, E-cadherin, β-catenin, occludin, claudin-1, claudin-3, and β-actin in C. B, H4 cells grown on chamber slides were treated with or without 0.1 unit/ml Epo for 24 h and then subjected to immunofluorescence staining for ZO-1 and nuclei. Images were acquired by confocal microscopy. Representative immunoblots from three experiments with similar results are shown. Data from three experiments are presented as mean ± S.E. relative to controls in percentage. * depicts statistical significance with a p value <0.05 by one-way ANOVA with Bonferroni correction..