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. 2011 Feb 1;286(14):12157–12165. doi: 10.1074/jbc.M110.198804

FIGURE 5.

FIGURE 5.

MSH3-deficient cells show a decrease in DNA double strand break repair efficiency. A, immunofluorescence staining for pH2AX foci formation in the HCT116+3+5, G5 with doxycycline (DOX) and G5 cells without doxycycline. The cells were treated with 5 μm oxaliplatin for 6 h and were analyzed by immunofluorescence after the indicated hours. B, inefficient decline of pH2AX-positive cells in the MSH3-deficient cells. C, immunofluorescence staining for 53BP1 foci formation in G5 with doxycycline and G5 cells without doxycycline. The cells were treated with 5 μm oxaliplatin for 6 h and were analyzed by immunofluorescence after 48 h. At least 100 cells were counted in each slide. Data are represented as mean ± S.E. from three or four independent experiments. The statistical difference between MSH3-deficient and -proficient G5 was determined by a two-sided Student's t test. * and *** represent p < 0.05 and p < 0.001, respectively.