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. 2011 Feb 16;286(14):12213–12220. doi: 10.1074/jbc.M110.214619

FIGURE 2.

FIGURE 2.

Neutralization of TLR2 or TLR4, but not LOX-1, reduces oxLDL-induced BMP-2 expression. A and B, representative immunoblots of three separate experiments and densitometric data show that neutralization of TLR2 or TLR4 markedly reduced BMP-2 levels after oxLDL stimulation (80 μg/ml, 24 h). C and D, representative immunoblots of three separate experiments and densitometric data show that PGN (10 μg/ml, 24 h) and LPS (200 ng/ml, 24 h) also induced BMP-2 expression and that their effects were greatly reduced by neutralizing antibodies against the receptors. E, representative immunoblot of three separate experiments and densitometric data show that neutralization of LOX-1 had no influence on oxLDL-induced BMP-2 expression (80 μg/ml oxLDL, 24 h). Densitometry data are expressed as mean ± S.E. (error bars). n = 3; *, p < 0.05 versus control; #, p < 0.05 versus correspondent treatment without receptor neutralization.