FIGURE 6.

Chromatin immunoprecipitation assay detects Stat1 binding to the Duox2 promoter. Starved BxPC-3 cells were treated with solvent or 25 ng/ml of IFN-γ for 1 or 24 h; cells were then harvested and used for the ChIP assay. Input lanes verifying equal amounts of DNA were used for the initial immunoprecipitation; control IgG- and Stat1-specific antibodies were used to pull down DNA, which was then extracted and used for PCR with primers spanning the potential Stat1 binding site in the Duox2 promoter. The resulting PCR products were separated on a 2% agarose gel and visualized with ethidium bromide staining.