FIGURE 3.
Affinity values for TatGGG interaction with import carriers. A, the KD* value for TatGGG interaction with Impα is plotted against the calculated Impα cytoplasmic concentration. Two average values of affinity are clearly distinguishable; KD* ∼ 26 μm for low Impα levels (∼1 μm), where the endogenous Impβ may play a role in modulating Impα autoinhibition (schematic); KD* ∼ 175 μm for Impα levels >10 μm, where the contribution of endogenous Impβ can be considered negligible. B, in the case of the TatGGG interaction with Impβ, we find a broad distribution of affinity values (plot) but with no clear dependence on Impβ expression levels. This can be explained by the lack of any possible modulation for the direct interaction to Impβ carrier (schematic).