FIGURE 4.

Thr²⁸⁶ autophosphorylation time courses of αCaMKII activated by wild type and mutant CaMs. A–G, Western blots (shown in H) for CaM (A), CaM1 (B), CaM2 (C), CaM3 (D), CaM4 (E), CaM12 (F), and CaM34 (G) were analyzed by densitometry as described under ”Materials and Methods“ and by fitting the inverted relative density to an exponential function. The deduced rate constants and relative amplitudes are shown in Table 2. H, Western blots of Thr²⁸⁶ autophosphorylation time courses were stimulated by wild type CaM, CaM1, CaM2, CaM3, CaM4, CaM12, and CaM34, as indicated. The error bars represent the S.D. from three sets of data.