TABLE 1.

Substrate specificity of the galactosyltransferase CgtD from RM1221 and GC149, measured using FCHASE-reducing end glycan derivatives (at 0.5 mm) and determined by CE

CgtD from RM1221 promotes efficient α1,4-Gal transfer to LacNAc (and Lac), matching its LOS substrate, but it is not capable of promoting transfer to a terminal Galβ1,3-GalNAcβ acceptor (GM1a), the preferred substrate for CgtD in GC149.

Acceptor (−FCHASE)		Specific activity
		CgtD-RM1221	CgtD-GC149
		units/mg
Lac-	Galβ-1,4-Glc-	0.40	0.54
LacNAc-	Galβ-1,4-GlcNAc-	0.60	0.51
GM1a-	Galβ-1,3-GalNAcβ-1,4-[NeuAcα-2,3-]-Galβ-1,4-Glc-	0.00	0.75