TABLE 2.

Substrate specificity of the sialyltransferase CstII from RM1503 measured using FCHASE-reducing end glycan derivatives (at 0.5 mm) and determined using CE

CstII from RM1503 promotes LNB sialylation with greater than 5-fold more efficiency than terminal Gal-β1,3-GalNAcβ (GM1a) and Gal-β1,4-Glcβ (Lac) acceptors, which are natural substrates for the enzyme in strains expressing ganglioside mimics.

Acceptor (−FCHASE)		Specific activity
		milliunits/mg
LNB-	Galβ-1,3-GlcNAc-	111.9
GM1a-	Galβ-1,3-GalNAcβ-1,4-[NeuAcα-2,3-]-Galβ-1,4-Glc-	17.5
Lac-	Galβ-1,4-Glc-	16.8
LacNAc	Galβ-1,4-GlcNAc-	6.6
3′Sialyl-Lac-	NeuAcα-2,3-Galβ-1,4-Glc-	0.1