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Incorporation of pBPA into SecA protein. The secA59(Am) mutant was grown in the presence or absence of pBPA and/or isopropyl-β-d-thiogalactopyranoside (IPTG) as indicated. Cell cultures were grown and analyzed as described under “Experimental Procedures.” Western blotting with Ni+2-conjugated horseradish peroxidase is shown. The position of SecA protein is given.
