FIGURE 2.

Specificity of SecA·SecY photocross-linking. The indicated secA and secY plasmid-bearing strain was grown and subjected to photocross-linking (+) or not (−), and cells were analyzed as described in “Experimental Procedures.” Western blotting utilized either SecA antisera (A) or c-Myc antibody (B). The positions of SecA, the SecA·SecY cross-linked complex, and molecular weight markers are given. Only the upper portion of the 20-cm-long gel utilized for better separation of high molecular weight proteins was transferred by electroblotting.