FIGURE 2.

NMR-derived structures of Ca²⁺-free Ncs1 and comparison with recoverin (Protein Data Bank code 1iku) and GCAP1 (Protein Data Bank code 2rlr). A, superimposed ensemble of NMR structures for Ca²⁺-free, myristoylated Ncs1. Overall r.m.s.d. is 0.69 Å for main chain atoms (see Table 1). EF-hands and myristoyl group (magenta) are colored as defined in Fig. 1. Ribbon diagrams of Ca²⁺-free myristoylated Ncs1 (B), recoverin (Protein Data Bank code 1iku) (C), and GCAP1 (Protein Data Bank code 2r2i) (D) are shown. Close-up views of the myristate binding pocket in Ncs1 (E) and recoverin (F) are shown.