TABLE 1.
Structural statistics for Ca2+-free Ncs1
| Myristoylated | Unmyristoylated | |
|---|---|---|
| NMR restraints | ||
| Short range NOE (i to i + j, j = 1–4) | 1010 | 506 |
| Long range NOE (i to i + j, j >4) | 446 | 268 |
| Hydrogen bonds | 124 | 124 |
| Dihedral angle restraints | 200 | 200 |
| Protein-myristate | 18 | |
| r.m.s.d. to the mean coordinates | ||
| Backbone of structured regionsa | 0.69 ± 0.05 Å | 0.85 ± 0.09 Å |
| Heavy atoms of structured regions | 1.17 ± 0.08 Å | 1.34 ± 0.1 Å |
| r.m.s.d. from idealized geometry | ||
| Bond lengths | 0.0064 ± 0.0001 Å | 0.0059 ± 0.0001 Å |
| Bond angles | 2.00 ± 0.0014° | 2.00 ± 0.0014° |
| Impropers | 0.9 ± 0.005° | 0.9 ± 0.005° |
| Ramachandran statistics of 15 structures | ||
| Most favored regions | 85% | 82% |
| Additional allowed regions | 12% | 15% |
| Generously allowed regions | 2% | 3% |
| Disallowed regions | 1% | 0% |
a Pairwise r.m.s.d. was calculated among 15 refined structures: residues in regions of regular secondary structure (10–20, 25–37, 42–55, 62–72, 79–89, 98–108, 115–130, 146–156, 163–174, 178–183).