TABLE 2.
Structural statistics for the Ca2+-bound Ncs1-Pik1 complex
| Ncs1 | Pik1(111–159) | |
|---|---|---|
| NMR restraints | ||
| Short range NOE (i to i + j, j = 1–3) | 715 | 112 |
| Long range NOE (i to i + j, j > 3) | 250 | 62 |
| Hydrogen bonds | 124 | 38 |
| Calcium bound at EF2, EF3, and EF4 | 18 | |
| Dihedral angle restraints | 200 | 54 |
| Intermolecular distance restraints | 66 | |
| r.m.s.d. to the mean coordinates | ||
| Backbone of structured regionsa | 0.9 ± 0.07 Å | 0.9 ± 0.09 Å |
| Heavy atoms of structured regions | 1.4 ± 0.08 Å | 1.4 ± 0.1 Å |
| r.m.s.d. from idealized geometry | ||
| Bond lengths | 0.007 ± 0.0001 Å | 0.009 ± 0.0001 Å |
| Bond angles | 2.07 ± 0.04° | 2.09 ± 0.04° |
| Impropers | 0.9 ± 0.05° | 0.95 ± 0.05° |
| Ramachandran statistics of 15 structures | ||
| Most favored regions | 76% | 80% |
| Additional allowed regions | 17% | 15% |
| Generously allowed regions | 6% | 4% |
| Disallowed regions | 1% | 1% |
a Residues in regions of regular secondary structure: Ncs1, residues 9–16, 24–37, 42–55, 62–72, 79–92, 99–108, 115–129, 145–156, 163–175; Pik1, residues 113–125, 144–156.