FIGURE 2.

Sortilin overexpression increases sAPPβ and secreted Aβ. A, HEK293 cells stably expressing either V5-tagged empty vector (vector) or V5-tagged sortilin (sort-V5) were compared via Western blotting. A, top two panels, representative data showing lysate subjected to SDS-PAGE and immunoblotted with anti-V5 or anti-APP. Full-length APP levels are shown (m, mature APP; im, immature APP). A, bottom two panels, immunoprecipitation of sAPPβ and Aβ from conditioned media. B, infrared quantification of immunoprecipitated sAPPβ. C, chemiluminescent quantification of immunoprecipitated total Aβ. Medium quantifications are normalized to total protein. Each bar represents the average of two experiments assayed in triplicate (lanes 1–3 of A). **, p < 0.01. Error bars, S.D.