FIGURE 4.

Co-immunoprecipitation analysis of exogenously and endogenously expressed sortilin and BACE1. A, Western blot analysis of co-immunoprecipitation input. HeLa cells were transiently co-transfected with sortilin (sort) and/or HA-tagged BACE1 (BACE1-HA). Negative controls include a mock transfection (no), sortilin transfection alone, and BACE1-HA transfection alone. B, co-immunoprecipitation (IP) results. Cells were lysed and subjected to co-immunoprecipitation using anti-sortilin or anti-HA. Samples were resolved via Western blotting using anti-HA and anti-sortilin, respectively. *, IgG. C, co-immunoprecipitation of HEK293 lysate by anti-BACE1 antibody. Negative controls include no antibody (beads) and a control antibody (mouse-derived) against an unrelated protein. Samples were resolved via Western blotting using anti-sortilin. D, human post-mortem brain tissue was co-immunoprecipitated with either no antibody (beads) or anti-BACE1. Samples were resolved via Western blotting using anti-sortilin.