FIGURE 1.

Schematic representations of the domain organization of TraI and the TraI constructs used in this study. The N-terminal relaxase domain contains residues 1–309; helicase domain contains residues 310–1476, and the CTD contains residues 1477–1504. The asterisk indicates the location of the catalytic residue Lys-998. RecD-like domain I (residues 303–844) and RecD-like domain II (residues 830–1473) identified by sequence analysis (17) are highlighted by dark lines. TraI constructs are shown in open boxes, whose first and last residues are indicated.