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. 2011 Feb 2;286(14):12670–12682. doi: 10.1074/jbc.M110.207563

TABLE 2.

Thermodynamic parameters of TraI binding to ssDNA oligomers of length ranging from 19 to 31 nucleotides in standard DNA binding buffer at 25 °C

The following abbreviations are used: n, stoichiometry; KN, macroscopic binding constant; Kint, intrinsic binding constant; NA, not applicable.

T19 T21 T23 T25 T27 T29 T31
n 1 1 1 1 1 1 1
KN ×107 (m−1) 0.9 ± 0.1 1.6 ± 0.1 2.5 ± 0.1 2.6 ± 0.3 6.2 ± 0.3 10 ± 1 12 ± 1
Kint × 107 (m−1) NA NA NA 2.6 ± 0.3 2.1 ± 0.1 2.1 ± 0.1 1.8 ± 0.5